J curcas tissue culture have been confined to leaflet, petiole, cotyledon, hypocotyl and axillary bud however, the regeneration frequencies, especially adventitious bud inductive frequencies, were still low hence protocols for in vitro propagation of j curcas through leaf and petiole explants with high frequency ( 85 %) have. Crops and plants have been considered for the purpose, among these, jatropha curcas, a member of reserves in this context, non-edible vegetable oil of jatropha curcas has the potential of providing a promising and the objective of this study was to develop an in vitro plant regeneration method, from leaf disc explants. 1h) callus and shoot induction from epicotyls and hypocotyls the best multiple shoot formation and callus induc- tion from both epicotyl and hypocotyl explants was obtained on culture medium supplemented with ba fig 1 plant regeneration from various explants of jatropha curcas l a,b: axillary buds c,d: shoot tips. Ahmed saad attaya, danny geelen, abd el-fatah helmy belal progress in jatropha curcas tissue efficient tissue culture protocols for direct and callus mediated shoot regeneration and somatic embryogenesis key words: jatropha curcas plant tissue culture micropropagation in vitro rooting organogenesis explant. Jatropha curcas, micropropagation, explant, désinfection, embryogenèse somatique, culture de tissu in vitro culture of jatropha curcas l the extension of jatropha curcas l cultivation as a biofuel feedstock species requires the distribution of a elle correspond à la régénération des plantes entières par développement.
In vitro shoot regeneration from leaf disc cultures jatropha curcas, an important biofuel plant mubashar nazir mintoo1, rao dg 2 and dantu p k1 1department of botany, faculty of science, dayalbagh educational institute, dayalbagh agra 2department of mechanical engineering,. Micropropagation of j curcas from node explants this protocol is economically more profitable keywords: jatropha curcas l, bud aggregates, nodal explant, regeneration, in vitro culture introduction among the plant species producing raw materials for biofuels, jatropha curcas l is one of the plant. Full-text paper (pdf): optimized in vitro plant regeneration of the biodiesel plant jatropha curcas l: the effects of using seeds at different stages of maturity as starting materials. That are current obstacles to in vitro culturing and manipulation this study was performed with the objective of evaluating the effects of different explants, growth regulators, coconut water, nitrogenous compounds and the sealing of flasks in promoting the regeneration of adventitious shoots in jatropha curcas (l) results.
Jatropha curcas, the energy plant has attained great attention in recent years because of its biodiesel production potential however, oil and deoiled cakes are toxic a non-toxic variety of j curcas. Must develop a superior genotype that can provide high and stable seed yields in degraded and marginal lands genetic engineering is a practical technique to improve jatropha that will complement conventional breeding for successful genetic modification, efficient in vitro plant regeneration and stable.
In vitro celldevbiol- plant (2013) 49:529-540 doi 1 01007/s 11 627-0 13- 9530-z micropropagation factors influencing direct shoot regeneration from mature leaves of jatropha curcas , an important biofuel plant chao zhang • shaoping fu • gaijuan tang • xinwen hu • jianchun guo received. Etc control straight adventitious manifold shoot bud regeneration from various explants such as petiole, hypocotyl, and cotyledon of j curcas [5,7,9,10] jatropha can be easily propagated by conventional means and highly cross pollinated but variations among species are limited and heterozygous seeds that decreases oil. Factors influencing in vitro regeneration through direct shoot bud induction from hypocotyl explants of jatropha curcas were studied in the present investigation regeneration in j curcas was found to be genotype dependent and out of four toxic and one non-toxic genotype studied, non-toxic was least responsive the best. Thus, the development of efficient techniques to ensure its safe conservation and regeneration is therefore of paramount importance in this work we describe a method for jatropha curcas seeds cryoexposure and seedling recovery after thawed in a first experiment, an efficient protocol for in vitro plant recovery was carried.
In vitro regeneration is vital for seedling system for mass production key words: plant growth regulators, callus, shoot induction, root formation introduction the genus jatropha, which is perceived to comprise approximately 170 known species, is distributed in the tropical and subtropical world. An efficient and reproducible method for the regeneration of jatropha curcas plants with a tap root system has been developed an in vitro shoot tip grafting technique was adopted to increase recovery of non-rooting shoots developed in culture the in vitro grafting success rate was dependent on a number.
Plant regeneration through the direct induction of shoot buds from petiole explants of jatropha curcas: a biofuel plant the orientation (horizontal or vertical) and source (in vitro or in vivo) of explants also significantly influenced plant regeneration the elongated shoots could be rooted on half-strength ms. The effect of cytokinins and auxins was evaluated in inducing a physic nut ( jatropha curcas l) callus while the effect of the indole-3-acetic acid (iaa) gave the highest shoot regeneration percentage and shoot height from the callus on the other hand, the in vitro seedlings of 'phetchaburi' physic nut by culturing seeds. An efficient and reproducible protocol for induction of adventitious shoot buds and plant regeneration from petiole explant cultures of jatropha curcas, an important biofuel crop, is described physiologically mature trees of three j curcas genotypes were selected and explants were prepared from young. There have been reports of successful regeneration of both plant species in vitro jatropha plantlets have been successfully regenerated using hypocotyl, nodal cuttings, peduncle and leaf explants (sujatha and reddy, 2000 rajore and batra , 2005 ahn et al, 2007 ahn and chen, 2008) similarly, there are reports of.
Tissue culture studies for development of regeneration protocol for jatropha 20 introduction jatropha curcas is a drought resistant shrub belonging to the genus euphorbiaceae it has been touted as a biodiesel plant whole plants through in vitro regeneration from the transformed tissue 21 literature studies. Indian journal of biotechnology vol 6, october 2007, pp 545-548 an alternative source for regenerable organogenic callus induction in jatropha curcas l shilpa rajore1 plant regeneration of jatropha curcas was achieved through organogenesis in callus cultures in vitro micropropagation and genetic improvement. For rooting of in vitro-cultivated j curcas in particular, the balance and application of synergistic compounds keywords in vitro root induction jatropha curcas light-emitting diodes (leds) phloroglucinol effect of four plant growth regulators on shoot regeneration from shoot tips explants of j curcas. And poverty alleviation within marginalized communities to meet large scale demand and ensure continuous supply of elite planting material there is need to establish clonal seed orchards and develop mass production techniques through tissue culture protocols for high frequency in vitro regeneration of jatropha.